|
摘要：
|
|
摘要：目的 探讨白藜芦醇对肝癌细胞干性的抑制作用。方法 采用细胞毒性实验检测不
同浓度（0、20、40、80、160、320 μmol/L）白藜芦醇处理HepG2及Huh7细胞48 h后的细
胞活力。采用平板克隆实验及细胞划痕实验检测不同浓度（0、20、40、60 μmol/L）白
藜芦醇对HepG2及Huh7细胞增殖及迁移的影响。采用Western blot实验检测白藜芦醇对肝
癌相关肿瘤干细胞标志物CD44及CD133表达的影响。结果 不同浓度（0、20、40、80、
160、320 μmol/L）白藜芦醇处理48 h后，HepG2细胞活力分别为100%、（93.43 ± 1.56）%、
（78.46 ± 2.63）%、（68.39 ± 2.42）%、（61.69 ± 3.65）%和（11.71 ± 1.22）%，Huh7
细胞活力分别为100%、（83.67 ± 4.51）%、（73.39 ± 3.14）%、（65.15 ± 4.78）%、
（55.33 ± 3.21）%、（31.38 ± 2.49）%；HepG2及Huh7细胞的IC50分别为145.7 μmol/L和
159.1 μmol/L。划痕实验表明，随白藜芦醇浓度升高，HepG2细胞及Huh7细胞迁移率逐渐
下降。平板克隆实验表明，不同药物浓度（0、20、40、60 μmol/L）白藜芦醇处理后，
HepG2克隆数分别为（264.64 ± 7.57）个、（129.00 ± 11.53）个、（33.67 ± 6.03）个、0个，
Huh7细胞克隆数分别为（173.67 ± 6.03）个、（115.00 ± 16.7）个、（24.00 ± 5.29）个、
0个，差异均有统计学意义（F值分别为744.2、227.4，P均＜ 0.001）。Western blot实验
表明，不同浓度（0、20、40、60 μmol/L）白藜芦醇均可抑制HepG2及Huh7中CD44及
CD133蛋白的表达，且随药物浓度增高，其抑制效果更明显。结论 白藜芦醇可抑制肝
癌细胞的增殖及迁移能力，还可抑制肝癌细胞相关干性标志物CD44及CD133的表达。
|
|
Abstract: Objective To investigate the inhibitory effect of resveratrol on hepatocellular
carcinoma cell stemness. Methods The proliferation capacity of HepG2 and Huh7 cells
were measured by cytotoxicity assay after resveratrol treating at different concentrations
(0, 20, 40, 80, 160, 320 μmol/L) for 48 hours. The effects of resveratrol on the proliferation
and migration of HepG2 and Huh7 cells were evaluated via colony formation assay and cell
scratch assay at concentrations of 0, 20, 40, and 60 μmol/L. Western blot was performed
to detect the expression levels of liver cancer-related cancer stem cell markers CD44 and
CD133 upon resveratrol treatment. Results After treatment with resveratrol at different
concentrations (0, 20, 40, 80, 160, 320 μmol/L) for 48 hours, the viability of HepG2 cells was
100%, (93.43 ± 1.56)%, (78.46 ± 2.63)%, (68.39 ± 2.42)%, (61.69 ± 3.65)% and (11.71 ±
1.22)%, respectively, and the viability of Huh7 cells was 100%, (83.67 ± 4.51)%, (73.39 ±
3.14)%, (65.15 ± 4.78)%, (55.33 ± 3.21)% and (31.38 ± 2.49)%, respectively. IC50 values
for HepG2 and Huh7 cells were 145.7 μmol/L and 159.1 μmol/L, respectively. Scratch assay
showed that the migration rate of HepG2 and Huh7 cells gradually decreased with resveratrol
concentration increasing. Colony formation assay demonstrated that after treatment with
resveratrol at concentrations of 0, 20, 40, and 60 μmol/L, the number of HepG2 colonies
was 264.64 ± 7.57, 129.00 ± 11.53, 33.67 ± 6.03 and 0, respectively, while the number of
Huh7 cell colonies was 173.67 ± 6.03, 115.00 ± 16.7, 24.00 ± 5.29 and 0, respectively. The
differences were statistically significant (F = 744.2, 227.4; both P ＜ 0.001). Western blot
analysis indicated that resveratrol at different concentrations (0, 20, 40, 60 μmol/L) inhibited
the expression of CD44 and CD133 proteins in both HepG2 and Huh7 cells, with a more
pronounced inhibitory effect as the drug concentration increased. Conclusion Resveratrol can
inhibit the proliferation and migration abilities of liver cancer cells, and can also suppress the
expression of liver cancer-related stemness markers CD44 and CD133.
|
|
基金项目：
|
|
|
|
作者简介：
|
|
|
|
参考文献：
|
|
|
|
服务与反馈：
|
|
【文章下载】【加入收藏】
|
|
|
|